ENDO-DEOXYRIBONUCLEASE FROM STREPTOMYCES-RIMOSUS

被引：5

作者：

VUKELIC, B

RITONJA, A

VITALE, L

机构：

[1] RUDJER BOSKOVIC INST,DEPT ORGAN CHEM & BIOCHEM,ZAGREB 41001,CROATIA

[2] JOZEF STEFAN INST,DEPT BIOCHEM & MOLEC BIOL,LJUBLJANA 61001,SLOVENIA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1995年 / 43卷 / 06期

关键词：

D O I：

10.1007/BF00166925

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

From filtrates of an oxytetracycline-producing culture of Streptomyces rimosus a deoxyribonuclease was purified to homogeneity and determined to be a potent endo-DNase. It is a monomeric, basic protein (M(r) approximate to 21000; pI approximate to 9.5) stable in a broad pH range but unstable to higher temperature. The enzyme has an absolute requirement for Mg2+ or Mn2+, and for its full activity requires free SH groups and a low-ionic-strength environment. Its N-terminal primary structure differs from that of other nucleases.

引用

页码：1056 / 1060

页数：5

共 24 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].