IMPROVEMENT OF THE THERMOSTABILITY OF PYRUVATE DECARBOXYLASE BY MODIFICATION WITH AN AMYLOSE DERIVATIVE

被引：0
|
作者：
OHBA, H [1 ]
YASUDA, S [1 ]
HIROSUE, H [1 ]
YAMASAKI, N [1 ]
机构：
[1] KYUSHU UNIV, FAC AGR, BIOCHEM LAB, HIGASHI KU, FUKUOKA 812, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 08期
关键词：
D O I：
10.1271/bbb.59.1581
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pyruvate decarboxylase from brewer's yeast was modified with the N-hydroxysuccinimide ester of an amylose glycylglycine adduct and some properties of the resulting conjugate were studied with regard to thermostability. By this conjugation the optimum temperature of the activity of pyruvate decarboxylase shifted from 35 degrees C to 40 degrees C. The conjugate showed a greater resistance than the enzyme to inactivating by heat treatment. It is suggested that AG-ONSu can be advantageously used for stabilization of the thermolabile enzyme.
引用
收藏
页码：1581 / 1583
页数：3
相关论文
共 50 条
  • [1] YEAST PYRUVATE DECARBOXYLASE - MODIFICATION OF TYROSINE AND LYSINE RESIDUES
    WEIDNERKLEINE, B
    ULLRICH, J
    HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1977, 358 (10): : 1294 - 1295
  • [2] Recent advances in the improvement of enzyme thermostability by structure modification
    Xu, Zhe
    Cen, Yu-Ke
    Zou, Shu-Ping
    Xue, Ya-Ping
    Zheng, Yu-Guo
    CRITICAL REVIEWS IN BIOTECHNOLOGY, 2020, 40 (01) : 83 - 98
  • [3] PYRUVATE DECARBOXYLASE DEFICIENCY IN LIVER
    WILLEMS, JL
    MONNENS, LAH
    TRIJBELS, JM
    SENGERS, RCA
    VEERKAMP, JH
    NEW ENGLAND JOURNAL OF MEDICINE, 1974, 290 (07): : 406 - 407
  • [4] ORIGIN OF PYRUVATE IN HISTIDINE DECARBOXYLASE
    RILEY, DW
    SNELL, EE
    FEDERATION PROCEEDINGS, 1969, 28 (02) : 346 - &
  • [5] PYRUVATE DECARBOXYLASE IN PHOTOSYNTHETIC BACTERIA
    QADRI, SMH
    HOARE, DS
    BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 148 (01) : 304 - &
  • [6] REGULATION OF CORN PYRUVATE DECARBOXYLASE
    Lee, T. C.
    Langston-Unkefer, P. J.
    PLANT PHYSIOLOGY, 1983, 72 : 125 - 125
  • [7] MODIFICATION OF AMYLOSE WITH DIKETENE
    ARRANZ, F
    SANCHEZCHAVES, M
    RIOFRIO, A
    MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS, 1986, 187 (11): : 2591 - 2598
  • [8] Role of pyruvate in enhancing pyruvate decarboxylase stability towards benzaldehyde
    Rosche, B
    Breuer, M
    Hauer, B
    Rogers, PL
    JOURNAL OF BIOTECHNOLOGY, 2005, 115 (01) : 91 - 99
  • [9] Improvement of cellulase stability by the covalent modification of copolymer of polyalkylene derivative
    Park, JW
    BIOTECHNOLOGY TECHNIQUES, 1996, 10 (06) : 457 - 462
  • [10] INHIBITION OF TRANSKETOLASE AND PYRUVATE DECARBOXYLASE BY OMEPRAZOLE
    NIXON, PF
    DIEFENBACH, RJ
    DUGGLEBY, RG
    BIOCHEMICAL PHARMACOLOGY, 1992, 44 (01) : 177 - 179
12345