IDENTIFICATION OF A GIBBERELLIN-INDUCED GENE IN DEEP-WATER RICE USING DIFFERENTIAL DISPLAY OF MESSENGER-RNA

被引：30
|
作者：
VANDERKNAAP, E [1 ]
KENDE, H [1 ]
机构：
[1] MICHIGAN STATE UNIV,US DOE,PLANT RES LAB,E LANSING,MI 48824
来源：
PLANT MOLECULAR BIOLOGY | 1995年 / 28卷 / 03期
关键词：
DIFFERENTIAL DISPLAY; HISTONE H3; DEEP-WATER RICE (ORYZA SATIVA L.); S PHASE-SPECIFIC EXPRESSION;
D O I：
10.1007/BF00020405
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Differential display of mRNA was employed to identify gibberellin (GA)-regulated genes in deepwater rice. One of the first differentially displayed products identified was shown to be ten-fold induced after start of GA treatment. The sequence of the clone shows complete amino acid identity with histone H3, and its increased mRNA level correlates with the onset of DNA synthesis. We also identified a gene whose expression pattern did not change over the course of treatment with GA and can be used as standard to correct for loading differences on northern blots.
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页码：589 / 592
页数：4
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