ISOLATION AND CHARACTERIZATION OF A MULTIENZYME COMPLEX CONTAINING DNA REPLICATIVE ENZYMES FROM MITOCHONDRIA OF SACCHAROMYCES-CEREVISIAE - MULTIENZYME COMPLEX FROM YEAST MITOCHONDRIA

被引：6

作者：

MURTHY, V ^{[1
]}

PASUPATHY, K ^{[1
]}

机构：

[1] BHABHA ATOM RES CTR,DIV RADIAT BIOL & BIOCHEM,BOMBAY 400085,MAHARASHTRA,INDIA

来源：

MOLECULAR BIOLOGY REPORTS | 1995年 / 20卷 / 03期

关键词：

MITOCHONDRIA; MULTIENZYME COMPLEX; REPLICATION; SACCHAROMYCES CEREVISIAE;

D O I：

10.1007/BF00990545

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 40 S multienzyme complex containing mtDNA polymerase was isolated from mitochondria of S. cerevisiae by density gradient centrifugation and by gel filtration chromatography. Besides DNA polymerase, RNA polymerase, primase, 3'-->5' exonuclease and an ATPase activities were found to be associated with it. The presence of some of these enzymes were confirmed by Western blot. This high molecular weight multienzyme complex containing DNA has most of the attributes of a putative replisome.

引用

页码：135 / 141

页数：7

共 26 条

[1] CHARACTERIZATION OF A MICROSOMAL SUBFRACTION ASSOCIATED WITH MITOCHONDRIA OF THE YEAST, SACCHAROMYCES-CEREVISIAE - INVOLVEMENT IN SYNTHESIS AND IMPORT OF PHOSPHOLIPIDS INTO MITOCHONDRIA
GAIGG, B
SIMBENI, R
HRASTNIK, C
PALTAUF, F
DAUM, G
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1234 (02): : 214 - 220
[2] Purification and characterization of a soluble polyphosphatase from mitochondria of Saccharomyces cerevisiae
Lichko, LP
Kulakovskaya, TV
Kulaev, IS
BIOCHEMISTRY-MOSCOW, 2000, 65 (03) : 355 - 360
[3] ISOLATION AND CHARACTERIZATION OF CARNITINE ACETYLTRANSFERASE FROM SACCHAROMYCES-CEREVISIAE
KISPAL, G
CSEKO, J
ALKONYI, I
SANDOR, A
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1085 (02) : 217 - 222
[4] PURIFICATION OF FERRICHROME SYNTHETASE FROM ASPERGILLUS-QUADRICINCTUS AND CHARACTERIZATION AS A PHOSPHOPANTETHIENE CONTAINING MULTIENZYME COMPLEX
SIEGMUND, KD
PLATTNER, HJ
DIEKMANN, H
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1076 (01) : 123 - 129
[5] EXPRESSION IN SACCHAROMYCES-CEREVISIAE OF A GENE ASSOCIATED WITH CYTOPLASMIC MALE-STERILITY FROM MAIZE - RESPIRATORY DYSFUNCTION AND UNCOUPLING OF YEAST MITOCHONDRIA
GLAB, N
WISE, RP
PRING, DR
JACQ, C
SLONIMSKI, P
MOLECULAR & GENERAL GENETICS, 1990, 223 (01): : 24 - 32
[6] CHARACTERIZATION OF MITOCHONDRIAL-DNA PRIMASE FROM SACCHAROMYCES-CEREVISIAE
MURTHY, V
PASUPATHY, K
JOURNAL OF BIOSCIENCES, 1994, 19 (01) : 1 - 8
[7] THE MULTIENZYME COMPLEX CONTAINING 9 AMINOACYL-TRANSFER-RNA SYNTHETASES IS UBIQUITOUS FROM DROSOPHILA TO MAMMALS
KERJAN, P
CERINI, C
SEMERIVA, M
MIRANDE, M
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1994, 1199 (03): : 293 - 297
[8] Kinetic characterization of the rotenone-insensitive internal NADH:: Ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae
Velázquez, I
Pardo, JP
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 389 (01) : 7 - 14
[9] Isolation and characterization of triacylglycerol-secreting mutant strain from yeast, Saccharomyces cerevisiae
Nojima, Y
Kibayashi, A
Matsuzaki, H
Hatano, T
Fukui, S
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1999, 45 (01) : 1 - 6
[10] NADP-DEPENDENT ALCOHOL DEHYDROGENASES IN BACTERIA AND YEAST - PURIFICATION AND PARTIAL CHARACTERIZATION OF THE ENZYMES FROM ACINETOBACTER-CALCOACETICUS AND SACCHAROMYCES-CEREVISIAE
WALES, MR
FEWSON, CA
MICROBIOLOGY-SGM, 1994, 140 : 173 - 183

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