COMPOUND D609 INHIBITS PHORBOL ESTER-STIMULATED PHOSPHOLIPASE-D ACTIVITY AND PHOSPHOLIPASE C-MEDIATED PHOSPHATIDYLETHANOLAMINE HYDROLYSIS

被引：60
|
作者：
KISS, Z
TOMONO, M
机构：
[1] The Hormel Institute, University of Minnesota, Austin, MN 55912
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1259卷 / 01期
关键词：
D609; PHOSPHOLIPASE D; PHORBOL ESTER; FIBROBLAST;
D O I：
10.1016/0005-2760(95)00148-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tricyclodecan-9-yl-xanthogenate (compound D609) has recently been used in various cellular systems to specifically inhibit the activity of phosphatidylcholine (PtdCho)-directed phospholipase C (PLC). Here we show that in intact NIH 3T3 fibroblasts, concentrations of D609 (35 to 50 mu g/ml) which have been used to inhibit PLC activity also significantly inhibit phorbol ester-induced phospholipase D-mediated hydrolysis of both PtdCho and phosphatidylethanolamine (PtdEtn). In addition, in isolated membranes compound D609 also inhibited PLC-mediated PtdEtn hydrolysis. The results indicate that compound D609 cannot be considered as a specific inhibitor of PtdCho-directed PLC activity.
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页码：105 / 108
页数：4
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