ISOLATION OF OVINE BETA-LACTOGLOBULIN GENETIC-VARIANTS BY CHROMATOFOCUSING - HETEROGENEITY OF BETA-LACTOGLOBULIN-A

被引：3

作者：

FERNANDEZESPLA, MD ^{[1
]}

LOPEZGALVEZ, G ^{[1
]}

RAMOS, M ^{[1
]}

机构：

[1] INST FERMENTACIONES IND,JUAN CIERVA 3,E-28006 MADRID,SPAIN

来源：

CHROMATOGRAPHIA | 1993年 / 37卷 / 1-2期

关键词：

CHROMATOFOCUSING; BETA-LACTOGLOBULIN; GENETIC VARIANTS; EWES MILK;

D O I：

10.1007/BF02272186

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatofocusing was applied to the isolation of ovine beta-Lactoglobulin (beta-Lg) genetic variants. Ovine beta-Lg A and B, which are known to differ only by a single amino-acid substitution, were separated. Moreover, one component differing from A variant in isoelectric point and relative molecular mass has been isolated in samples containing ovine beta-Lg A. Although elution of beta-Lgs occurred far from their ''normal'' isoelectric points, chromatofocusing is a powerful technique effective for both analytical and preparative scale.

引用

页码：43 / 46

页数：4