DETECTION OF IRON-BINDING PROTEINS BY A BLOTTING TECHNIQUE

被引：13

作者：

CHEN, Y

DRYSDALE, J

机构：

[1] Department of Biochemistry, Tufts University School of Medicine, Boston

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 212卷 / 01期

关键词：

D O I：

10.1006/abio.1993.1289

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple method for detecting iron binding proteins based on binding of 59Fe to protein blots from SDS gels followed by autoradiography. This binding is specific and is not affected by other metal ions. The method has allowed identification of new iron binding proteins in intestinal mucosa and in Hela cells. © 1994 Academic Press, Inc. All rights reserved.

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页码：47 / 49

页数：3

相关论文

共 11 条

[1] IRON TRANSPORT AND STORAGE PROTEINS [J].

AISEN, P ;

LISTOWSKY, I .

ANNUAL REVIEW OF BIOCHEMISTRY, 1980, 49 :357-393

[2]

AOKI Y, 1986, ANAL BIOCHEM, V157, P117, DOI 10.1016/0003-2697(86)90204-6

[3]

CONRAD ME, 1990, J BIOL CHEM, V265, P5273

[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[5] DETECTION OF CALCIUM-BINDING PROTEINS BY CA-45 AUTORADIOGRAPHY ON NITROCELLULOSE MEMBRANE AFTER SODIUM DODECYL-SULFATE GEL-ELECTROPHORESIS [J].

MARUYAMA, K ;

MIKAWA, T ;

EBASHI, S .

JOURNAL OF BIOCHEMISTRY, 1984, 95 (02) :511-519

[6] ZINC-BINDING PROTEINS DETECTED BY PROTEIN BLOTTING [J].

MAZEN, A ;

GRADWOHL, G ;

DEMURCIA, G .

ANALYTICAL BIOCHEMISTRY, 1988, 172 (01) :39-42

[7] CHARACTERIZATION OF A ZINC BLOTTING TECHNIQUE - EVIDENCE THAT A RETROVIRAL GAG PROTEIN BINDS ZINC [J].

SCHIFF, LA ;

NIBERT, ML ;

FIELDS, BN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) :4195-4199

[8] IDENTIFICATION OF ZINC-BINDING SITES OF PROTEINS - ZINC BINDS TO THE AMINO-TERMINAL REGION OF TUBULIN [J].

SERRANO, L ;

DOMINGUEZ, JE ;

AVILA, J .

ANALYTICAL BIOCHEMISTRY, 1988, 172 (01) :210-218

[9] IRON UPTAKE BY RAT DUODENAL MICROVILLOUS MEMBRANE-VESICLES - EVIDENCE FOR A CARRIER MEDIATED TRANSPORT-SYSTEM [J].

STREMMEL, W ;

LOTZ, G ;

NIEDERAU, C ;

TESCHKE, R ;

STROHMEYER, G .

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1987, 17 (02) :136-145

[10] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354