IDENTIFICATION OF ROSE CULTIVARS BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM

被引：29
|
作者：
RAJAPAKSE, S [1 ]
HUBBARD, M [1 ]
KELLY, JW [1 ]
ABBOTT, AG [1 ]
BALLARD, RE [1 ]
机构：
[1] CLEMSON UNIV,DEPT BIOL SCI,CLEMSON,SC 29634
来源：
SCIENTIA HORTICULTURAE | 1992年 / 52卷 / 03期
基金：
美国国家科学基金会;
关键词：
CULTIVAR IDENTIFICATION; DNA ANALYSIS; GENETIC MARKERS; PLANT PATENT PROTECTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; ROSAXHYBRIDA; ROSE;
D O I：
10.1016/0304-4238(92)90024-7
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Restriction fragment length polymorphisms (RFLP) were investigated as a means of identifying rose (Rosa x hybrida) cultivars for patent protection. A library of genomic DNA was generated by shotgun cloning HindIII digested DNA fragments of the rose cultivar 'Confection' into the plasmid pUC8. Clones with low copy number sequences were identified and used as probes in Southern hybridization analyses of restriction digested DNA from different rose cultivars. We have identified 15 probes that display RFLP useful in cultivar identification. One of these probes, pH 1.3C, can identify 12 different cultivars. Based on hybridizations with probes pH 1.3C and pH 1.6C, a total of 16 cultivars have been distinguished from one another. Using different restriction enzyme and probe combinations, we expect to distinguish many more rose cultivars.
引用
收藏
页码：237 / 245
页数：9
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