IMMUNOLOGICAL CHARACTERIZATION OF THE ENDOPROTEASE-PC1 AND ENDOPROTEASE-PC2 IN ADRENAL CHROMAFFIN GRANULES AND IN THE PITUITARY-GLAND

被引：61

作者：

KIRCHMAIR, R

GEE, P

HOGUEANGELETTI, R

LASLOP, A

FISCHERCOLBRIE, R

WINKLER, H

机构：

[1] UNIV INNSBRUCK,DEPT PHARMACOL,PETER MAYR STR 1A,A-6020 INNSBRUCK,AUSTRIA

[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED,BRONX,NY 10461

来源：

FEBS LETTERS | 1992年 / 297卷 / 03期

基金：

美国国家卫生研究院;

关键词：

ENDOPROTEASE; PC1; PC2; CHROMAFFIN GRANULE; ADRENAL MEDULLA; PITUITARY;

D O I：

10.1016/0014-5793(92)80560-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific antisera against synthetic fragments of the endoproteases, PC1 and PC2, were used to characterize these proteins. In one-dimensional immunoblots these antisera labelled components of 85 kDa for PC1 and of 70 kDa for PC2 in purified bovine chromaffin granules and anterior and posterior pituitary of ox and rat. In membranes of bovine chromaffin granules glycoprotein H was identified as the major PC2 immunoreactive spot. A major part of these endoproteases appeared membrane bound.

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页码：302 / 305

页数：4

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