FIBER OPTIC SENSOR FOR CA2+ BASED ON AN INDUCED CHANGE IN THE CONFORMATION OF THE PROTEIN CALMODULIN

被引：23
作者：
BLAIR, TL
YANG, ST
SMITHPALMER, T
BACHAS, LG
机构：
[1] UNIV KENTUCKY,DEPT CHEM,LEXINGTON,KY 40506
[2] UNIV SOUTH,DEPT CHEM,SEWANEE,TN 37375
[3] ST FRANCIS XAVIER UNIV,DEPT CHEM,ANTIGONISH B2G 1C0,NS,CANADA
来源：
ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 02期
关键词：
D O I：
10.1021/ac00074a019
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A fiber optic sensor that exploits the natural selectivity of the Ca2+-binding protein calmodulin (CaM) is described. In this sensor, a dialysis membrane is used to entrap a fluorescein-labeled CaM (F-CaM) solution at the common end of a bifurcated fiber optic bundle. Ca2+ ions in a sample solution can diffuse through the membrane and bind to the F-CaM. Upon binding with Ca2+, CaM undergoes a conformational change that induces a change in the fluorescence of the attached fluorescein tag. This change in fluorescence can be related to the concentration of Ca2+ in the sample solution. The detection limit for the sensor is 5 x 10(-8) M Ca2+. The sensor has no interference by Mg2+ at concentrations as high as 10(-2) M.
引用
收藏
页码：300 / 302
页数：3
相关论文
共 29 条
[1]   FLUORESCENCE MONITORING OF A BENZO[A]PYRENE METABOLITE USING A REGENERABLE IMMUNOCHEMICAL-BASED FIBEROPTIC SENSOR [J].
ALARIE, JP ;
BOWYER, JR ;
SEPANIAK, MJ ;
HOYT, AM ;
VO-DINH, T .
ANALYTICA CHIMICA ACTA, 1990, 236 (02) :237-244
[2]  
[Anonymous], 1975, PURE APPL CHEM, V48, P129
[3]   FIBER OPTIC AMMONIA GAS SENSING PROBE [J].
ARNOLD, MA ;
OSTLER, TJ .
ANALYTICAL CHEMISTRY, 1986, 58 (06) :1137-1140
[4]   AN OPTICAL CALCIUM-ION SENSOR [J].
ASHWORTH, DC ;
HUANG, HP ;
NARAYANASWAMY, R .
ANALYTICA CHIMICA ACTA, 1988, 213 (1-2) :251-257
[5]   STRUCTURE OF CALMODULIN REFINED AT 2.2 A RESOLUTION [J].
BABU, YS ;
BUGG, CE ;
COOK, WJ .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (01) :191-204
[6]   FLUOROCARBON-BASED IMMOBILIZATION OF A FLUOROIONOPHORE FOR PREPARATION OF FIBER OPTIC SENSORS [J].
BLAIR, TL ;
CYNKOWSKI, T ;
BACHAS, LG .
ANALYTICAL CHEMISTRY, 1993, 65 (07) :945-947
[7]   EUROPIUM(III) LUMINESCENCE AND TYROSINE TO TERBIUM(III) ENERGY-TRANSFER STUDIES OF INVERTEBRATE (OCTOPUS) CALMODULIN [J].
BRUNO, J ;
HORROCKS, WD ;
ZAUHAR, RJ .
BIOCHEMISTRY, 1992, 31 (31) :7016-7026
[8]  
CHAO SH, 1984, MOL PHARMACOL, V26, P75
[9]   OPTICAL SENSOR FOR CALCIUM - PERFORMANCE, STRUCTURE, AND REACTIVITY OF CALCICHROME IMMOBILIZED AT AN ANIONIC POLYMER FILM [J].
CHAU, LK ;
PORTER, MD .
ANALYTICAL CHEMISTRY, 1990, 62 (18) :1964-1971
[10]   A CALCIUM-SELECTIVE OPTRODE BASED ON THE FLUORESCENCE OF DANSYLATED TROPONIN [J].
ECKERTTILOTTA, SE ;
SCOUTEN, WH ;
HINES, J .
APPLIED SPECTROSCOPY, 1991, 45 (03) :491-495
123