TRANSFORMING GROWTH-FACTOR-BETA INHIBITS THE CYTOKINE-MEDIATED EXPRESSION OF THE INDUCIBLE NITRIC-OXIDE SYNTHASE MESSENGER-RNA IN HUMAN RETINAL-PIGMENT EPITHELIAL-CELLS

被引：35
|
作者：
KUTTY, RK [1 ]
KUTTY, G [1 ]
HOOKS, JJ [1 ]
WIGGERT, B [1 ]
NAGINENI, CN [1 ]
机构：
[1] NEI,IMMUNOL LAB,BETHESDA,MD 20892
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 01期
关键词：
D O I：
10.1006/bbrc.1995.2477
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human retinal pigment epithelial (RPE) cells in culture respond to a mixture of cytokines (IFN-gamma, IL-1 beta, TNF-alpha) by producing large amounts of nitric oxide. Transforming growth factor-beta, unlike other growth factors, was found to inhibit this response by more than 75%. The expression of mRNA for the inducible form of nitric oxide synthase in RPE cells treated with cytokines was demonstrated by reverse transcription-polymerase chain reaction, sequencing of the PCR product and northern blotting. Transforming growth factor-beta was highly effective in inhibiting (by 75%) the cytokine-induced nitric oxide synthase mRNA expression. This response by RPE may play an important role in the etiology of infectious and inflammatory diseases affecting retina. (C) 1995 Academic Press, Inc.
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页码：386 / 393
页数：8
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