PANCREATIC BETA-CELL REPLICATION AND AMELIORATION OF SURGICAL DIABETES BY REG PROTEIN

被引：229

作者：

WATANABE, T

YONEMURA, Y

YONEKURA, H

SUZUKI, Y

MIYASHITA, H

SUGIYAMA, K

MORIIZUMI, S

UNNO, M

TANAKA, O

KONDO, H

BONE, AJ

TAKASAWA, S

OKAMOTO, H

机构：

[1] TOHOKU UNIV, SCH MED, DEPT BIOCHEM, AOBA KU, SENDAI, MIYAGI 980, JAPAN

[2] TOHOKU UNIV, SCH MED, DEPT ANAT, AOBA KU, SENDAI, MIYAGI 980, JAPAN

[3] KANAZAWA UNIV, SCH MED, DEPT SURG, KANAZAWA, ISHIKAWA 920, JAPAN

[4] UNIV BRIGHTON, DEPT PHARM, BRIGHTON BN2 4GJ, E SUSSEX, ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 09期

关键词：

REG GENE; DIABETES MELLITUS; ISLETS OF LANGERHANS; GROWTH FACTOR;

D O I：

10.1073/pnas.91.9.3589

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We previously isolated from a rat regenerating islet cDNA library a gene named Reg, which is expressed in regenerating islets but is not expressed in normal islets. Here we examined the effect of rat Reg protein on pancreatic beta-cell replication using both 90% depancreatized rats and isolated islets. The depancreatized rats that received i.p. administration of recombinant rat Reg protein (1 mg/kg per day) for 2 months showed amelioration of the surgical diabetes, as evidenced by a significant decrease in blood glucose with an increased beta cell mass in the residual pancreas, In isolated rat islets, Reg protein (18-180 nM: 0.3-3 mu g/ml) significantly increased [H-3]thymidine incorporation into the nuclei of beta cells. These results indicate that Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus.

引用

页码：3589 / 3592

页数：4

共 21 条

[1] N-TERMINAL SEQUENCE EXTENSION IN THE GLYCOSYLATED FORMS OF HUMAN PANCREATIC STONE PROTEIN - THE 5-OXOPROLINE N-TERMINAL CHAIN IS O-GLYCOSYLATED ON THE 5TH AMINO-ACID RESIDUE [J].