EFFECT OF A TEST MEAL, WITHOUT AND WITH PROTEIN, ON MUSCLE AND PLASMA-FREE AMINO-ACIDS

被引:67
作者
BERGSTROM, J
FURST, P
VINNARS, E
机构
[1] UNIV HOHENHEIM, INST BIOL CHEM & NUTR, W-7000 STUTTGART 70, GERMANY
[2] ST GORANS UNIV HOSP, METAB RES LAB, S-11281 STOCKHOLM, SWEDEN
[3] ST GORANS UNIV HOSP, INTENS CARE UNIT, S-11281 STOCKHOLM, SWEDEN
关键词
Amino acids; Muscle; Plasma; Protein-free meal; Protein-rich meal;
D O I
10.1042/cs0790331
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
1. The effect of a protein-free meal and a protein-rich meal on the concentration of free amino acids in plasma and muscle tissue was studied in eight healthy subjects. The energy content of the protein-free meal was 3800 kJ. The protein-rich meal was identical in composition except that 50 g of bovine serum albumin was added. Plasma and samples from the quadriceps femoris muscle (percutaneous muscle biopsy) for amino acid determination were collection before and at 1, 3, 5 and 7 h after the meal. 2. After the protein-free meal the concentrations of most essential amino acids and of some non-essential amino acids in plasma decreased continuously below basal levels at 5-7 h. The muscle concentration of essential amino acids fell too, reaching its nadir 3-5 h after the meal. The decrease in plasma amino acid concentration was smaller than the decrease in muscle concentration for all essential amino acids except phenylalanine. 3. The concentrations of most amino acids in plasma increased transiently 1 and 3 h after the protein-rich meal; histidine and several non-essential amino acids fell below the basal levels at 5-7 h after the meal. In muscle, threonine, valine, leucine, lysine and alanine were increased at 1 and 3 h after the protein-rich meal; isoleucine, serine and glycine fell below the basal level after 5 and 7 h. For the essential amino acids except threonine and lysine, the increase in plasma concentration was greater than the increase in muscle concentration. 4. Correlations were observed between the relative content of the essential amino acids in the bovine serum albumin and the increase in concentration of these amino acids in plasma and muscle. Methionine and isoleucine, which had the lowest content in bovine serum albumin, fell below basal levels in plasma and (for isoleucine) in muscle 5-7 h after the meal, suggesting that these two amino acids might have been limiting for protein synthesis. 5. Amino acid analysis in plasma and muscle samples taken postprandially should be evaluated with caution considering the strong influence of meal composition on plasma and muscle free amino acids profiles.
引用
收藏
页码:331 / 337
页数:7
相关论文
共 19 条
[1]   AMINO-ACID LEVELS IN PLASMA, LIVER, AND SKELETAL-MUSCLE DURING PROTEIN DEPRIVATION [J].
ADIBI, SA ;
MODESTO, TA ;
MORSE, EL ;
AMIN, PM .
AMERICAN JOURNAL OF PHYSIOLOGY, 1973, 225 (02) :408-414
[2]   PROTEIN DIGESTION IN HUMAN INTESTINE AS REFLECTED IN LUMINAL, MUCOSAL, AND PLASMA AMINO-ACID CONCENTRATIONS AFTER MEALS [J].
ADIBI, SA ;
MERCER, DW .
JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (07) :1586-1594
[3]   INFLUENCE OF HYPERINSULINEMIA ON INTRACELLULAR AMINO-ACID LEVELS AND AMINO-ACID EXCHANGE ACROSS SPLANCHNIC AND LEG TISSUES IN UREMIA [J].
ALVESTRAND, A ;
DEFRONZO, RA ;
SMITH, D ;
WAHREN, J .
CLINICAL SCIENCE, 1988, 74 (02) :155-163
[4]   PLASMA AMINO-ACID RESPONSES IN HUMANS TO EVENING MEALS OF DIFFERING NUTRITIONAL COMPOSITION [J].
ASHLEY, DV ;
BARCLAY, DV ;
CHAUFFARD, FA ;
MOENNOZ, D ;
LEATHWOOD, PD .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1982, 36 (01) :143-153
[5]   INCREASE IN ANTERIOR TIBIALIS MUSCLE PROTEIN-SYNTHESIS IN HEALTHY MAN DURING MIXED AMINO-ACID INFUSION - STUDIES OF INCORPORATION OF [1-C-13]LEUCINE [J].
BENNET, WM ;
CONNACHER, AA ;
SCRIMGEOUR, CM ;
SMITH, K ;
RENNIE, MJ .
CLINICAL SCIENCE, 1989, 76 (04) :447-454
[6]   INABILITY TO STIMULATE SKELETAL-MUSCLE OR WHOLE-BODY PROTEIN-SYNTHESIS IN TYPE-1 (INSULIN-DEPENDENT) DIABETIC-PATIENTS BY INSULIN-PLUS-GLUCOSE DURING AMINO-ACID INFUSION - STUDIES OF INCORPORATION AND TURNOVER OF TRACER L-[1-C-13]LEUCINE [J].
BENNET, WM ;
CONNACHER, AA ;
SMITH, K ;
JUNG, RT ;
RENNIE, MJ .
DIABETOLOGIA, 1990, 33 (01) :43-51
[7]   INFLUENCE OF SEVERE POTASSIUM-DEPLETION AND SUBSEQUENT REPLETION WITH POTASSIUM ON MUSCLE ELECTROLYTES, METABOLITES AND AMINO-ACIDS IN MAN [J].
BERGSTROM, J ;
ALVESTRAND, A ;
FURST, P ;
HULTMAN, E ;
SAHLIN, K ;
VINNARS, E ;
WIDSTROM, A .
CLINICAL SCIENCE AND MOLECULAR MEDICINE, 1976, 51 (06) :589-599
[8]   INTRACELLULAR FREE AMINO-ACID CONCENTRATION IN HUMAN MUSCLE-TISSUE [J].
BERGSTROM, J ;
FURST, P ;
NOREE, LO ;
VINNARS, E .
JOURNAL OF APPLIED PHYSIOLOGY, 1974, 36 (06) :693-697
[9]  
BERGSTROM J, 1962, SCAND J CLIN LAB INV, V14, P1
[10]  
BROWN JR, 1975, FED PROC, V312, P431