IDENTIFICATION OF PHOSPHOPEPTIDES BY MASS-SPECTROMETRY

被引：22

作者：

SCHROEDER, W ^{[1
]}

COVEY, T ^{[1
]}

HUCHO, F ^{[1
]}

机构：

[1] SCIEX LTD,DIV MDS HLTH GRP LTD,THORNHILL L3T 1P2,ONTARIO,CANADA

来源：

FEBS LETTERS | 1990年 / 273卷 / 1-2期

关键词：

Acetylcholine receptor; Mass spectrometry; Protein phosphorylation;

D O I：

10.1016/0014-5793(90)81044-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphopeptides can be identified by ion spray mass spectrometry. The method was tested with phosphokemptide and with a proteolytic digest of one subunit (δ-subunit) of the nicotinic acetylcholine receptor. In the latter one peptide containing tyrosine phosphate, one with two serine phosphates, and two different peptides each containing one serine phosphate were unambiguously identified. Thus it is proven that ion spray mass spectrometry can be applied for the localization of phosphorylation sites in a known primary structure. © 1990.

引用

页码：31 / 35

页数：5

共 11 条

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