CALCIUM-DEPENDENT TRANSMITTER SECRETION RECONSTITUTED IN XENOPUS OOCYTES - REQUIREMENT FOR SYNAPTOPHYSIN

被引：127
作者：
ALDER, J
LU, B
VALTORTA, F
GREENGARD, P
POO, MM
机构：
[1] COLUMBIA UNIV,DEPT BIOL SCI,NEW YORK,NY 10027
[2] ROCKEFELLER UNIV,MOLEC & CELLULAR NEUROSCI LAB,NEW YORK,NY 10021
[3] UNIV MILAN,NATL RES COUNCIL,CTR CYTOPHARMACOL,DEPT PHARMACOL,I-20129 MILAN,ITALY
来源：
SCIENCE | 1992年 / 257卷 / 5070期
关键词：
D O I：
10.1126/science.1353905
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Calcium-dependent glutamate secretion was reconstituted in Xenopus oocytes by injecting the oocyte with total rat cerebellar messenger RNA (mRNA). Co-injection of total mRNA with antisense oligonucleotides to synaptophysin message decreased the expression of synaptophysin in the oocyte and reduced the calcium-dependent secretion. A similar effect on secretion was observed for oocytes injected with total mRNA together with an antibody to rat synaptophysin. These results indicate that synaptophysin is necessary for transmitter secretion and that the oocyte expression system may be useful for dissecting the molecular events associated with the secretory process.
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页码：657 / 661
页数：5
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