GENETIC-MAP OF RHIZOBIUM-MELILOTI MEGAPLASMID PRMESU47B

被引：55
作者：
CHARLES, TC [1 ]
FINAN, TM [1 ]
机构：
[1] MCMASTER UNIV,DEPT BIOL,1280 MAIN ST W,HAMILTON L8S 4K1,ONTARIO,CANADA
来源：
JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 05期
关键词：
D O I：
10.1128/jb.172.5.2469-2476.1990
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A circular linkage map of the Rhizobium meliloti megaplasmid pRmeSU47b was constructed. The map consists of transposon insertions carrying alternating antibiotic resistance markers linked by φM12 transduction. Data from conjugation experiments utilizing donor strains carrying Tn5-oriT insertions in the megaplasmid supported the proposed genetic map. In addition, the positions of previously identified Fix, exopolysaccharide synthetic, thiamine synthetic, and C4-dicarboxylate transport loci on the megaplasmid map were determined. By converting cotransduction frequencies to physical distance, we calculated the replicon to be 1,600 kilobases in size, which compares favorably with previous physical estimates.
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页码：2469 / 2476
页数：8
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