MONOCLONAL ANTIBODY-BASED IMMUNOLOCALIZATION OF BOUND TRIAZINE RESIDUES IN 2 AQUATIC MACROPHYTES (ELODEA-CANADENSIS AND MYRIOPHYLLUM-SPICATUM)

被引：5

作者：

GIERSCH, T

SOHN, G

HOCK, B

机构：

[1] Technical University of München, Department of Botany at Weihenstephan

来源：

ANALYTICAL LETTERS | 1993年 / 26卷 / 09期

关键词：

HERBICIDES; ATRAZINE; IMMUNOLOCALIZATION; MONOCLONAL ANTIBODIES; BOUND RESIDUES;

D O I：

10.1080/00032719308017434

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Immunohistochemical localization offers a fast and reliable method of obtaining information about the distribution of bound pesticide residues in plants. In the present study aquatic macrophytes (Elodea canadensis, Myriophyllum spicatum) were grown in laboratory model ecosystems spiked with 50 mug/l atrazine. To label cryosections, monoclonal antibodies from cell culture supernatants and ascites fluid with differing specificities for s-triazines were used in combination with the fluorescent dye Phycoerythrin and biotin-streptavidin amplification. The best results were observed with antibodies gained from mice immunized with an ametryn sulfoxide-BSA conjugate, regardless of the method employed for antibody production. The consequences with respect to herbicide metabolism and binding are discussed.

引用

页码：1831 / 1845

页数：15

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