SIMPLE AND EFFICIENT METHOD FOR ISOLATION AND MEASUREMENT OF CYANOBACTERIAL HEPATOTOXINS BY PLANT-TESTS (SINAPIS-ALBA L)

被引：118
作者：
KOS, P
GORZO, G
SURANYI, G
BORBELY, G
机构：
[1] LAJOS KOSSUTH UNIV, DEPT BOT, H-4010 DEBRECEN, HUNGARY
[2] BIOL RES CTR, INST PLANT BIOL, H-6701 SZEGED, HUNGARY
来源：
ANALYTICAL BIOCHEMISTRY | 1995年 / 225卷 / 01期
关键词：
D O I：
10.1006/abio.1995.1106
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard (Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to 95-97% microcystin without the need for an HPLC system and justifies the use of the S, alba L, seedling test in the quantitative assessment of the toxin with an IC50 Of 3 mu g ml-1 instead of the mouse intraperitoneal test. (C) 1995 Academic Press, Inc.
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页码：49 / 53
页数：5
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