A METHOD FOR CHROMOSOME PREPARATIONS FROM LARGE FISH SPECIMENS USING IN-VITRO SHORT-TERM TREATMENT WITH COLCHICINE

被引：159

作者：

FORESTI, F ^{[1
]}

OLIVEIRA, C ^{[1
]}

DEALMEIDATOLEDO, LF ^{[1
]}

机构：

[1] USP,INST BIOCIENCIAS,DEPT BIOL,SAO PAULO,BRAZIL

来源：

EXPERIENTIA | 1993年 / 49卷 / 09期

关键词：

FISH CYTOGENETICS; MITOTIC CHROMOSOMES; TECHNIQUE;

D O I：

10.1007/BF01923555

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This paper describes a new technique for preparing mitotic fish chromosomes using short-term in vitro treatment with colchicine. The results show that a large number of good quality metaphases (many suitable for chromosome banding) can be obtained by this technique, which requires an average of 1 h and 30 min for all steps. The procedure considerably reduces the time normally required for chromosome preparations in fish.

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页码：810 / 813

页数：4

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