USEFUL HYDROLYTIC ENZYMES - PROTEASES, LIPASES AND NITRILASES

被引：12

作者：

BERGER, B

DERAADT, A

GRIENGL, H

HAYDEN, W

HECHTBERGER, P

KLEMPIER, N

FABER, K

机构：

[1] Institute of Organic Chemistry, Graz University of Technology, Graz, Stremayrgasse 16

来源：

PURE AND APPLIED CHEMISTRY | 1992年 / 64卷 / 08期

关键词：

D O I：

10.1351/pac199264081085

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The most recent developments in the use of hydrolytic enzymes - proteases, lipases and nitrilases - are summarized: Sterically demanding alpha, alpha-disubstituted carboxylic esters can be enzymatically resolved using a commercially available protease derived from Aspergillus oryzae. A significant enhancement of selectivity and activity of Candida cylindracea lipase in irreversible acyl transfer reactions may be achieved by removal of by-products or via covalent modification of the enzyme. Enzymatic hydrolysis of nitriles proceeds under mild conditions but enzyme-catalysed side-reactions such as ester- or epoxide-hydrolysis may occur.

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页码：1085 / 1088

页数：4

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