Rapid detection of hemolysin gene of Klebsiella pneumoniae by Loop-Mediated Isothermal Amplification (LAMP)

被引：0

作者：

Pasupathi, Rathinasabapathi ^{[1
]}

机构：

[1] SRM Univ, Dept Genet Engn, Madras 6030203, Tamil Nadu, India

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2018年 / 13卷 / 04期

关键词：

Klebsiella pneumoniae; hemolysin; LAMP; Pneumonia;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Klebsiella pneumoniae is a wide-spread nosocomial pathogen that causes a broad range of diseases including pneumonia. In the current study, a loop-mediated isothermal amplification (LAMP) method was developed for a rapid and sensitive detection of K. pneumoniae by targeting hemolysin gene. The supplementation of additives such as dimethyl sulfoxide and betaine enhanced the efficiency of DNA amplification. The LAMP amplified the target gene with four specific primers in 45 min at 65 degrees C in a simple water bath. All the strains other than K. pneumoniae were found negative for LAMP detection which indicates a high specificity of LAMP reaction. The method was found to be sensitive up to one femtogram of genomic DNA which is 1000 times more than that of PCR. The rapidness and specificity of LAMP assay advocate the potential of the LAMP for early detection of K. pneumoniae in clinical and environmental samples.

引用

页码：24 / 28

页数：5

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