PRODUCTION OF L-DIHYDROXYPHENYLALANINE IN ESCHERICHIA-COLI WITH THE TYROSINE PHENOL-LYASE GENE CLONED FROM ERWINIA-HERBICOLA

被引：54

作者：

FOOR, F

MORIN, N

BOSTIAN, KA

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 09期

关键词：

D O I：

10.1128/AEM.59.9.3070-3075.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene (tutA) encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promoters were constructed. The enzyme was expressed at high levels in E. coli in the presence of isopropyl-beta-D-thiogalactopyranoside or lactose as an inducer. L-Dihydroxyphenylalanine was synthesized in high yield from catechol, pyruvate, and ammonia by induced cells.

引用

页码：3070 / 3075

页数：6

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