LOCALIZATION OF CARBOXYPEPTIDASE-I IN GERMINATING BARLEY-GRAIN

被引:17
作者
RANKI, H [1 ]
SOPANEN, T [1 ]
VOUTILAINEN, R [1 ]
机构
[1] VYSKUMMY USTAV HUTNICTV ZELEZA,BIOTECH LAB,ESPOO,FINLAND
关键词
D O I
10.1104/pp.93.4.1449
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Activity measurements and Northern blot hybridizations were used to study the temporal and spatial expression of carboxypeptidase I in germinating grains of barley (Hordeum vulgare L. cv Himalaya). In the resting grain no carboxypeptidase I activity was found in the aleurone layer, scutellum, or starchy endosperm. During germination high levels of enzyme activity appeared in the scutellum and in the starchy endosperm but only low activity was found in the aleurone layer. No mRNA for carboxypeptidase I was observed in the resting grain. By day 1 of germination the mRNA appeared in the scutellum where its level remained high for several days. In contrast, little mRNA was observed in the aleurone layer. These results indicate that the scutellum plays an important role in the production of carboxypeptidase I in germinating barley grain.
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页码:1449 / 1452
页数:4
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