EFFICIENT IN-SITU ELECTROPORATION OF MAMMALIAN-CELLS GROWN ON MICROPOROUS MEMBRANES

被引:15
作者
YANG, TA
HEISER, WC
SEDIVY, JM
机构
[1] YALE UNIV,SCH MED,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06520
[2] BIORAD LABS,DIV GENET SYST,HERCULES,CA 94547
关键词
D O I
10.1093/nar/23.15.2803
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electroporation is a common technique for the introduction of DNA molecules into living cells, The method is currently limited by the necessity of applying the electrical discharge to cells in suspension, Adherent cells must therefore be removed from their substratum, which can induce unwanted physiological effects, We report here a new procedure for in sitar electroporation of cells grown on microporous membranes of polyethylene terephthalate (PET) or polyester (PE). We demonstrate that this method of in sitar electroporation employs only readily available materials and standard electroporation devices without any modifications, is as efficient as conventional electroporation of cells in suspension, and is applicable to a wide range of cell types. Efficient electroporation can be achieved under conditions of minimal cell killing, and can be performed with quiescent cells as well as with confluent epithelial sheets. The method is a useful extension of electroporation technology, and will allow the application of electroporation to a wider spectrum of biological systems.
引用
收藏
页码:2803 / 2810
页数:8
相关论文
共 32 条