CHEMILUMINOMETRIC L-LYSINE DETERMINATION WITH IMMOBILIZED LYSINE OXIDASE BY FLOW-INJECTION ANALYSIS

被引：34

作者：

PREUSCHOFF, F

SPOHN, U

WEBER, E

UNVERHAU, K

MOHR, KH

机构：

[1] UNIV HALLE,INST BIOTECHNOL,WEINBERGWEG 16A,O-4050 HALLE,GERMANY

[2] UNIV HALLE,DEPT MED,INST BIOCHEM,O-4020 HALLE,GERMANY

[3] UNIV HALLE,INST MICROBIOL,O-4050 HALLE,GERMANY

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 280卷 / 02期

关键词：

CHEMILUMINESCENCE; ENZYMATIC METHODS; FLOW INJECTION; LYSINE;

D O I：

10.1016/0003-2670(93)85120-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzymatic flow-injection procedure for the determination of L-lysine was developed. A lysine oxidase reactor is combined with a fibre-optic hydrogen peroxide detector. Hydrogen peroxide detection is based on the peroxidase-catalysed luminol reaction. The chemiluminescent light is detected by a photomultiplier. L-Lysine can be determined in the range 10-1000 muM. A sampling rate of up to 90 h-1 can be achieved. The whole sensing assay works for more than 1 month. The double logarithmic graph of the peak signal height vs. the lysine concentration is linear, the slope being larger than unity (r2 = 0.991, n = 4).

引用

页码：185 / 189

页数：5

共 15 条

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