RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF THE DNA OF TYPHUS GROUP RICKETTSIAE

被引：0
作者：
ARTEMIEV, MI
IGNATOVICH, VF
RYDKINA, EB
LICHODED, LJ
BALAYEVA, NM
DEMKIN, VV
机构：
来源：
ACTA VIROLOGICA | 1991年 / 35卷 / 06期
关键词：
RICKETTSIAE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS; DNA; GENOTYPIC DIFFERENTIATION;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The DNA of 10 strains of Rickettsia prowazekii, 5 strains of Rickettsia typhi and 1 strain of Rickettsia canada was investigated by restriction fragment length polymorphism analysis. Interspecies differences were characterized by a great number of noncomigrating bands. Using the endonuclease HindIII and PstI fragments comigration as a quantitive criterion, genetic similarity coefficient was calculated for the pair Rickettsia prowazekii/Rickettsia typhi-32.0%, for Rickettsia prowazekii/Rickettsia canada-22.7 %, and for Rickettsia typhi/Rickettsia canada-23.5 %. Intraspecies differences expressed are very subtle and concern 1-2 noncomigrating fragments. The investigated strains of Rickettsia prowazekii and Rickettsia typhi can be divided into 2 groups without any correlation to the source and period of isolation, or to strain passage history.
引用
收藏
页码：526 / 530
页数：5
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