PURIFICATION AND CHARACTERIZATION OF A PROTEIN INHIBITOR OF CALCIUM-DEPENDENT PROTEASES FROM RAT-LIVER

被引：45

作者：

DEMARTINO, GN ^{[1
]}

CROALL, DE ^{[1
]}

机构：

[1] UNIV TEXAS, HLTH SCI CTR, DEPT PHYSIOL, DALLAS, TX 75235 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1984年 / 232卷 / 02期

关键词：

D O I：

10.1016/0003-9861(84)90592-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Soluble extracts of rat liver contain a protein inhibitor of Ca-dependent proteases. The inhibitor has an apparent MW = 250,000 and is separated from the Ca-dependent proteases by gel-filtration chromatography in the presence of EGTA [ethylene glycol bis (.beta.-aminoethyl ether)-N,N,N'',N''-tetroacetic acid]. The inhibitor was purified by affinity chromatography using a Ca-dependent protease covalently linked to Affi-Gel 15. The inhibitor specifically binds to this affinity resin in a Ca dependent manner and elutes in the presence of EDTA or EGTA. The purified inhibitor appears as a single protein with MW = 125,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Presumably it is a dimer under nondenaturing conditions. The inhibitor inhibits each of 2 Ca-dependent proteases from rat liver and from other tissues and species. However, it has no effect on any other protease tested.

引用

页码：713 / 720

页数：8

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