ENDOPLASMIC RETICULUM-TO-CYTOSOL TRANSPORT OF FREE POLYMANNOSE OLIGOSACCHARIDES IN PERMEABILIZED HEPG2 CELLS

Free polymannose oligosaccharides have recently been localized to both the vesicular and cytosolic compartments of HepG2 cells, Here we investigated the possibility that free oligosaccharides originating in the lumen of the endoplasmic reticulum (ER) are transported directly into the cytosol. Incubation of permeabilized cells in the absence of ATP at 37 degrees C led to the intravesicular accumulation of free Man(9)GlcNAc(2) which was generated from dolichol-linked oligosaccharide in the ER, This oligosaccharide remained stable within the permeabilized cells unless ATP was added to the incubations at which time the Man(9)GlcNAc(2) was partially converted to Man(8)GlcNAc(2), and both these components were released from an intravesicular compartment into the cytosolic compartment of permeabilized cells. In contrast, when permeabilized cells, primed with either free triglucosyloligosaccharide or a glycotripeptide, were incubated with ATP both these structures remained associated with the intravesicular compartment. As the conditions in which free oligosaccharides were transported out of the intravesicular compartment into the cytosolic compartment did not permit vesicular transport of glycoproteins from the ER to the Golgi apparatus our data demonstrate the presence of a transport process for the delivery of free polymannose oligosaccharides from the ER to the cytosol.