ESTIMATION OF RANOLAZINE AND 11 PHASE-I METABOLITES IN HUMAN PLASMA BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY WITH SELECTED-ION MONITORING

被引：34

作者：

HERRON, WJ ^{[1
]}

EADIE, J ^{[1
]}

PENMAN, AD ^{[1
]}

机构：

[1] SYNTEX RES CTR,EDINBURGH EH14 4AP,MIDLOTHIAN,SCOTLAND

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 712卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00475-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The estimation of ranolazine, a novel piperazine derivative, and eleven of its Phase I metabolites has been undertaken by liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry (LC-APCI-MS). Plasma samples, taken on day 5 of a multiple-dose study, were extracted by solid-phase extraction (SPE) and analysed, using a gradient HPLC system coupled to the APCI source of a Finnigan MAT TSQ 700 mass spectrometer. Metabolites were analysed in selected-ion monitoring (SIM) mode, using an instrument control language (ICL) procedure. The LC-MS combination allowed resolution of all eleven metabolites, including four hydroxylated metabolites and five unresolved components. The results from the linear regression showed good correlation (r(2) > 0.980) for all the metabolites. Plasma concentrations indicated that three metabolites were present at levels higher than 10% of the parent compound.

引用

页码：55 / 60

页数：6

共 6 条

[1] PROTECTIVE EFFECTS OF RANOLAZINE IN GUINEA-PIG HEARTS DURING LOW-FLOW ISCHEMIA AND THEIR ASSOCIATION WITH INCREASES IN ACTIVE PYRUVATE-DEHYDROGENASE [J].