11-BETA-HYDROXYSTEROID DEHYDROGENASE-ACTIVITY IN MESENTERIC-ARTERIES OF SPONTANEOUSLY HYPERTENSIVE RATS

被引：18

作者：

TAKEDA, Y ^{[1
]}

YONEDA, T ^{[1
]}

MIYAMORI, I ^{[1
]}

GATHIRAM, P ^{[1
]}

TAKEDA, R ^{[1
]}

机构：

[1] UNIV NATAL,SCH MED,DEPT PHYSIOL,DURBAN,SOUTH AFRICA

来源：

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | 1993年 / 20卷 / 10期

关键词：

GLUCOCORTICOID; 11-BETA-HYDROXYSTEROID DEHYDROGENASE; MESENTERIC ARTERY; NOREPINEPHRINE; SHR;

D O I：

10.1111/j.1440-1681.1993.tb01644.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. 11beta-Hydroxysteroid dehydrogenase (11-HSD) activity in mesenteric arteries of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats was determined and expressed as the percentage conversion of [H-3]-corticosterone to [H-3]-11-dehydrocorticosterone. 2. 11-HSD activity was significantly decreased in mesenteric arteries of both 4 and 9 week old SHR (8.4 +/- 0.8%, 5.0 +/- 1.5%, respectively) compared with WKY rats (12.4 +/- 0.6%, 15.8 +/- 0.7%, respectively; P < 0.05). 3. Total RNA from rat vascular smooth muscle cells (VSMC) and endothelial cells (EC) were prepared with selective precipitation in 3 mol/L LiCl/6 mol/L urea. The expression of 11-HSD mRNA was confirmed in the rat VSMC but its mRNA expression was not detected in EC, using northern blot analysis. 4. The results in this study indicate that 11-HSD in the vascular wall may play a role in the pathogenesis of hypertension in SHR.

引用

页码：627 / 631

页数：5

共 29 条

[21] MINERALOCORTICOID ACTIVITY OF CARBENOXOLONE - CONTRASTING EFFECTS OF CARBENOXOLONE AND LIQUORICE ON 11-BETA-HYDROXYSTEROID DEHYDROGENASE-ACTIVITY IN MAN [J].