EXPRESSION OF ACTIVE HUMAN INTERFERON-BETA IN TRANSGENIC PLANTS

被引:31
作者
EDELBAUM, O
STEIN, D
HOLLAND, N
GAFNI, Y
LIVNEH, O
NOVICK, D
RUBINSTEIN, M
SELA, I [1 ]
机构
[1] HEBREW UNIV JERUSALEM,FAC AGR,VIRUS LAB,IL-76100 REHOVOT,ISRAEL
[2] HEBREW UNIV JERUSALEM,FAC AGR,OTTO WARBURG CTR BIOTECHNOL,IL-76100 REHOVOT,ISRAEL
[3] AGR RES ORG,VOLCANI CTR,FAC GENET,IL-50250 BET DAGAN,ISRAEL
[4] WEIZMANN INST SCI,IL-76100 REHOVOT,ISRAEL
来源
JOURNAL OF INTERFERON RESEARCH | 1992年 / 12卷 / 06期
关键词
D O I
10.1089/jir.1992.12.449
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tobacco plants were transformed with the human gene for interferon-beta (IFN-beta). Transformation was determined by the polymerase chain reaction (PCR), and expression was determined by Western blot analysis, by purifying the IFN from the transgenic plants, and by bioassays indicating its activity in human cells. Plants expressing IFN-beta were self-pollinated. IFN-beta-expressing progeny plants were selected and produced active IFN-beta, indicating stable transformation.
引用
收藏
页码:449 / 453
页数:5
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