INFLAMMATORY MEDIATORS AND ACTIVITY OF HUMAN PERITONEAL-MACROPHAGES

被引：2

作者：

PRUIMBOOM, WM ^{[1
]}

VANDIJK, APM ^{[1
]}

TAK, CJAM ^{[1
]}

ZIJLSTRA, FJ ^{[1
]}

BONTA, IL ^{[1
]}

WILSON, JHP ^{[1
]}

机构：

[1] ACAD HOSP ROTTERDAM DIJKZIGT,DEPT INTERNAL MED 2,ROTTERDAM,NETHERLANDS

来源：

AGENTS AND ACTIONS | 1993年 / 38卷

关键词：

D O I：

10.1007/BF01991146

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Human peritoneal macrophages (hp-Mphi) are a source of inflammatory mediators. After stimulation in vitro for 24 h with LPS there was a significant increase in cytokine production (IL-1, IL-6 and TNFalpha), but not in the production of eicosanoids from endogenous arachidonate. Leukotrienes are the predominant eicosanoids formed after stimulation with calcium ionophore for 15 min, while prostaglandin formation is insignificant. The fluorescence intensity of TPA-stimulated and DHR123 loaded hp-Mphi (a measure of the respiratory burst) increases significantly in a short period of time. Hp-Mphi will be useful as a model for testing the effects of anti-inflammatory drugs on eicosanoid and cytokine production and respiratory burst activity in vitro.

引用

页码：C86 / C88

页数：3

共 4 条

[1] RESPIRATORY BURST ACTIVITY IN BRAIN MACROPHAGES - A FLOW CYTOMETRIC STUDY ON CULTURED RAT MICROGLIA [J].

BANATI, RB ;

ROTHE, G ;

VALET, G ;

KREUTZBERG, GW .

NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, 1991, 17 (03) :223-230

[2] A FAST AND EASY METHOD TO DETERMINE THE PRODUCTION OF REACTIVE OXYGEN INTERMEDIATES BY HUMAN AND MURINE PHAGOCYTES USING DIHYDRORHODAMINE-123 [J].

EMMENDORFFER, A ;

HECHT, M ;

LOHMANNMATTHES, ML ;

ROESLER, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) :269-275

[3]

GARRELDS IM, 1993, AGENTS ACTIONS, V38, pC89

[4]

PRUIMBOOM WM, 1993, 2ND P INT C EIC OTH, P541

← 1 →