EVOLUTION OF THE MITOCHONDRIAL RIBOSOMAL-RNA IN THE ORIENTAL SPECIES SUBGROUPS OF DROSOPHILA

被引：7
|
作者：
NIGRO, L
GRAPPUTO, A
机构：
[1] Dipartimento di Biologia, Universitá di Padova, 35121 Padova
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 1993年 / 21卷 / 01期
关键词：
MITOCHONDRIAL DNA; LARGE AND SMALL RIBOSOMAL-RNA GENES; DROSOPHILA SPECIES;
D O I：
10.1016/0305-1978(93)90011-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The nucleotide sequence of two segments of the mtDNA molecule of different species of Drosophila containing a portion of the large rRNA (16S) and a portion of the small rRNA (23S) has been determined. The polymerase chain reaction (PCR) technique with ''universal'' primers has been used to amplify the segments for direct sequencing. The species analysed belong to four different subgroups of the melanogaster species group. The phylogenetic relationships of the species have been reconstructed by using the neighbour-joining algorithm and the significance of the inferred phylogeny tested by bootstrap method.
引用
收藏
页码：79 / 83
页数：5
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