THEORETICAL AND EXPERIMENTAL ASPECTS OF INDIRECT DETECTION IN CAPILLARY ELECTROPHORESIS

被引：95

作者：

BRUIN, GJM ^{[1
]}

VANASTEN, AC ^{[1
]}

XU, XM ^{[1
]}

POPPE, H ^{[1
]}

机构：

[1] UNIV AMSTERDAM,ANALYT CHEM LAB,NIEUWE ACHTERGRACHT 166,1018 WV AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 608卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)87111-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Theoretical and experimental aspects of indirect UV detection are considered. Based on a mathematical treatment of the transport of ions through the capillary, resulting in an eigenvector-eigenvalue problem, some guidelines are formulated about how to increase efficiency and sensitivity in an indirect (UV) detection system. Also, the existence of system peaks can be explained properly. An experimental system consisting of seven amino acids as sample ions and salicylate at pH 11.0 as the UV-absorbing ion was chosen in order to compare theoretical and experimental results. Constructed electropherograms, produced with a computer program based on the above-mentioned mathematical treatment, are also presented and compared with experimental electropherograms.

引用

页码：97 / 107

页数：11

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