MULTIPLE FORMS OF INVERTASE FROM DAUCUS-CAROTA CELL-CULTURES

被引：27

作者：

STOMMEL, JR ^{[1
]}

SIMON, PW ^{[1
]}

机构：

[1] UNIV WISCONSIN,USDA ARS,1575 LINDEN DR,MADISON,WI 53706

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 07期

关键词：

carrot; Daucus carota; fructofuranosidase; invertase; isoenzymes; protein purification; Umbelliferae;

D O I：

10.1016/0031-9422(90)83010-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 400-fold purification of two isoenzymes of acid invertase (pH optima 4.5) and a two- to three-fold purification of alkaline (pH optima 7.5) invertase from suspension cultured carrot cells was obtained using ion-exchange (DEAE cellulose) and affinity (concanavalin-A) column chromatography. SDS polyacrylamide gel electrophoresis resolved major polypeptide bands at 61 000 and 49000 corresponding to the two isoenzymes of acid invertase, designated acid- 1 and acid-2, respectively. The acid invertase isoenzymes, in contrast with alkaline invertase, are likely glycoproteins based on their ability to bind the lectin concanavalin-A. © 1990.

引用

页码：2087 / 2089

页数：3

共 21 条

[1] A COLUMN METHOD FOR ENZYMIC CHARACTERIZATION OF COARSE CELLULAR FRACTIONS - APPLICATION TO INSOLUBLE BETA-FRUCTOFURANOSIDASE FROM GRAPE [J].

ARNOLD, WN .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1966, 113 (02) :451-&

[2]

AVIGAD G, 1982, ENCY PLANT PHYSL A, V13, P217

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] SUC GENES OF YEAST - A DISPERSED GENE FAMILY [J].

CARLSON, M ;

OSMOND, BC ;

BOTSTEIN, D .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1980, 45 :799-803

[5] ACCUMULATION OF SUCROSE IN VACUOLES ISOLATED FROM RED BEET TISSUE [J].

DOLL, S ;

RODIER, F ;

WILLENBRINK, J .

PLANTA, 1979, 144 (05) :407-411

[6] STUDIES ON BETA-FRUCTOSIDASE FROM RADISH SEEDLINGS .3. COMPARATIVE STUDIES ON SOLUBLE AND WALL-BOUND FORMS [J].

FAYE, L ;

GHORBEL, A .

PLANT SCIENCE LETTERS, 1983, 29 (01) :49-60

[7] STUDIES ON BETA-FRUCTOSIDASE FROM RADISH SEEDLINGS .1. PURIFICATION AND PARTIAL CHARACTERIZATION [J].

FAYE, L ;

BERJONNEAU, C ;

ROLLIN, P .

PLANT SCIENCE LETTERS, 1981, 22 (01) :77-87

[8] IMPROVED METHODOLOGY FOR ANALYSIS AND QUANTITATION OF PROTEINS ON ONE-DIMENSIONAL SILVER-STAINED SLAB GELS [J].

GIULIAN, GG ;

MOSS, RL ;

GREASER, M .

ANALYTICAL BIOCHEMISTRY, 1983, 129 (02) :277-287

[9] STRUCTURAL GENES OF INTERNAL INVERTASES IN SACCHAROMYCES-CEREVISIAE [J].

GROSSMANN, MK ;

ZIMMERMANN, FK .

MOLECULAR & GENERAL GENETICS, 1979, 175 (02) :223-229

[10] SUGAR ACCUMULATION CYCLE IN SUGAR CANE .1. STUDIES ON ENZYMES OF CYCLE [J].

HATCH, MD ;

GLASZIOU, KT ;

SACHER, JA .

PLANT PHYSIOLOGY, 1963, 38 (03) :338-+

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