INVESTIGATION ON THE DIFFERENCE EXPRESSION OF TYPE IV COLLAGEN alpha 1(IV)- alpha 6(IV) CHAIN MRNA IN NORMAL FIBROBLAST AND IN SKIN CELL MALIGNANT MELANOMA

Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains alpha 1 to alpha 6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important in order to provide more detailed understanding of the expression of collagen in tumor cells.. The aim of this study is to determine the alpha 1 to alpha 6 (IV) mRNA expression in the cell lines obtained from skin and melanoma cell. To investigate the mRNA expression, the RNA was extracted from the fibroblast and melanoma (A375) cell lines. The RNA was subjected to reverse transcription and then synthesized. The mRNA expression levels were measured using real time PCR with related to internal control, GAPDH. The study identified that alpha 1, alpha 2, alpha 4, alpha 5 and alpha 6 of the alpha 1-alpha 6 (IV) were expressed in skin fibroblast. This corresponds to the alpha 1 alpha 1 alpha 2 and a5 5 6 networks However, in melanoma cell lines the collagen IV alpha 2, alpha 4, alpha 5 and alpha 6 mRNA was observed in low level compared to alpha 1 and this suggested that the tumor has affected the expression of collagen and basement membrane of the cell.