DIFFERENTIAL AMPEROMETRIC DETERMINATION OF ALCOHOL IN BLOOD OR URINE USING ALCOHOL-DEHYDROGENASE

被引：42
|
作者：
SMITH, MD [1 ]
OLSON, CL [1 ]
机构：
[1] OHIO STATE UNIV,COLL PHARM,COLUMBUS,OH 43210
来源：
ANALYTICAL CHEMISTRY | 1975年 / 47卷 / 07期
关键词：
D O I：
10.1021/ac60357a076
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The differential amperometric method provides an inexpensive, easily automated method for measuring urine or whole blood alcohol concentrations. The method had the additional advantage of cycling NADH produced which prevents the reaction from coming to equilibrium. The reagent can be made several days prior to use since the diaphorase, NAD** plus , and DCPIP are stable in solution at 0-5 C for up to 1 month. The differential amperometric method has high sensitivity, is specific for ethanol, and can be successfully applied to routine analysis for samples of 10 to 20 mu l.
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页码：1074 / 1077
页数：4
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