A CARBON-PASTE ELECTRODE CHEMICALLY-MODIFIED WITH A PHENOTHIAZINE POLYMER DERIVATIVE FOR ELECTROCATALYTIC OXIDATION OF NADH - PRELIMINARY-STUDY

被引：32
作者：
DOMINGUEZ, E
LAN, HL
OKAMOTO, Y
HALE, PD
SKOTHEIM, TA
GORTON, L
机构：
[1] POLYTECH INST NEW YORK, DEPT CHEM, BROOKLYN, NY 11202 USA
[2] SUNY, MOLTECH CORP, STONY BROOK, NY 11794 USA
[3] LUND UNIV, CTR CHEM, DEPT ANALYT CHEM, S-22100 LUND, SWEDEN
来源：
BIOSENSORS & BIOELECTRONICS | 1993年 / 8卷 / 3-4期
关键词：
NADH; POLYMER MEDIATOR; MODIFIED CARBON PASTE ELECTRODE; FLOW INJECTION; AMPEROMETRY; ELECTROCATALYSIS;
D O I：
10.1016/0956-5663(93)85029-N
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A new kind of chemically modified electrode for electrocatalytic oxidation of NADH is described. Carbon paste (graphite : paraffin oil) electrodes are chemically modified with the addition of an aqueous insoluble polymer containing a covalently bound phenothiazine dye, Toluidine Blue O, through an amide linkage between a primary amine group of the Toluidine Blue O moiety and the polymer backbone. Some basic properties of this chemically modified carbon paste electrode for the electrocatalytic oxidation of NADH are described, using cyclic voltammetry, as are the properties when the electrode is used as an amperometric sensor for NADH in a flow injection system. At an applied potential of +100 mV vs. Ag/AgCl, the detection limit for NADH was 0.4 muM.
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页码：167 / 175
页数：9
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