PURIFICATION AND CHARACTERIZATION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE FROM ASPERGILLUS-NIGER AND ASPERGILLUS-NIDULANS

被引：17

作者：

WENNEKES, LMJ ^{[1
]}

GOOSEN, T ^{[1
]}

VANDENBROEK, PJM ^{[1
]}

VANDENBROEK, HWJ ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT GENET,DREIJENLAAN 2,6703 HA WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-11-2793

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Glucose-6-phosphate dehydrogenase (G6PD; D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1. 1. 1. 49) has been purified from Aspergillus nidulans and Aspergillus niger by a combination of affinity and anion exchange chromatography. A 500-1000-fold purification was obtained and the final enzyme preparations were shown to be pure but not homogeneous. For both fungi the purified enzyme preparation gave two bands on native and denaturing gels. The catalytically active form is a multimer. The molecular mass of the monomers is 60 and 57 kDa for A. nidulans and 55 and 53 kDa for A. niger. Both enzymes exhibited strict specificity towards both substrates glucose 6-phosphate and NADP+. The A. nidulans and A. niger G6PD enzymes catalyse the conversion of glucose 6-phosphate via a random order mechanism. Inhibition studies provided evidence for the physiological role of G6PD as producer of NADPH in both fungi.

引用

页码：2793 / 2800

页数：8

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