FUNCTIONAL EVIDENCE FOR AN APICAL ANP RECEPTOR IN LLC-PK1 KIDNEY EPITHELIAL-CELLS

被引：0
|
作者：
HASHIMOTO, Y
OZAKI, J
YASUHARA, M
HORI, R
SUGA, S
ITOH, H
NAKAO, K
INUI, K
机构：
[1] KYOTO UNIV HOSP,FAC MED,DEPT PHARM,SAKYO KU,KYOTO 606,JAPAN
[2] KINKI UNIV,INST PHARMACEUT RES & TECHNOL,OSAKA,OSAKA 577,JAPAN
[3] KYOTO UNIV,FAC MED,DEPT MED,DIV 2,SAKYO KU,KYOTO 606,JAPAN
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1994年 / 268卷 / 03期
关键词：
ANP (ATRIAL NATRIURETIC PEPTIDE); NATRIURETIC PEPTIDE; C-TYPE; GUANYLATE CYCLASE; LLC-PK1; CELL;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of alpha-human atrial natriuretic peptide (alpha-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with alpha-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for alpha-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.
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收藏
页码：443 / 445
页数：3
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