未找到相关数据
CLONING OF BOAR SPMI GENE WHICH IS EXPRESSED SPECIFICALLY IN SEMINAL-VESICLE AND CODES FOR A SPERM MOTILITY INHIBITOR PROTEIN
被引：3
|作者：
IWAMOTO, T
HIROAKI, H
FURUICHI, Y
WADA, K
SATOH, M
SATOH, M
OSADA, T
GAGNON, C
机构：
[1] NIPPON ROCHE RES CTR, KAMAKURA, KANAGAWA 247, JAPAN
[2] AGENE RES INST, KAMAKURA, KANAGAWA, JAPAN
[3] MCGILL UNIV, ROYAL VICTORIA HOSP, UROL RES LAB, MONTREAL, PQ H3A 1A1, CANADA
关键词：
BOAR SPMI;
SEMINAL PLASMA SPERM MOTILITY INHIBITOR;
SEMINAL VESICLE PROTEIN;
SEMINAL PLASMA;
DEMEMBRANATED-REACTIVATED SPERMATOZOA;
SPERMADHESIN;
D O I：
暂无
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
Boar semen contains a seminal plasma motility inhibitor (SPMI) that blocks the motility of demembranated-reactivated spermatozoa as well as of intact spermatozoa. In this paper, we describe the primary structure of SPMI, the coding of boar SPMI cDNA gene and its expression in various porcine tissues. Nucleotide sequence analysis of the 645-bp SPMI cDNA predicts a coded polypeptide of 137 amino acid residues which includes a 21-residue signal peptide and a 116-residue secreted protein. The amino acid sequence of SPMI was found to be highly homologous to AQN-3, a member of spermadhesin family proteins of boar that bind to spermatozoa. Expression of the boar SPMI gene detected by Northern blot analysis revealed that its expression is very abundant in seminal vesicles and specific to this tissue,
引用
收藏
页码：420 / 424
页数：5
相关论文