VISUALIZATION OF RNA TERTIARY STRUCTURE BY RNA-EDTA-CENTER-DOT-FE(II) AUTOCLEAVAGE - ANALYSIS OF TRNA(PHE) WITH URIDINE-EDTA-CENTER-DOT-FE(II) AT POSITION-47

被引：50
作者：
HAN, HY
DERVAN, PB
机构：
[1] Beckman Institute, California Institute of Technology, Pasadena
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 11期
关键词：
AFFINITY CLEAVING; YEAST TRNA(PHE); CLEAVAGE OF RNA;
D O I：
10.1073/pnas.91.11.4955
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA.Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)(2)(SO4)(2) and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.
引用
收藏
页码：4955 / 4959
页数：5
相关论文
共 35 条
[1]   LEAD-CATALYZED CLEAVAGE OF YEAST TRANSFER RNAPHE MUTANTS [J].
BEHLEN, LS ;
SAMPSON, JR ;
DIRENZO, AB ;
UHLENBECK, OC .
BIOCHEMISTRY, 1990, 29 (10) :2515-2523
[2]   CRYSTALLOGRAPHIC AND BIOCHEMICAL INVESTIGATION OF THE LEAD(II)-CATALYZED HYDROLYSIS OF YEAST PHENYLALANINE TRANSFER-RNA [J].
BROWN, RS ;
DEWAN, JC ;
KLUG, A .
BIOCHEMISTRY, 1985, 24 (18) :4785-4801
[3]  
DECH TR, 1990, ANNU REV BIOCHEM, V59, P543
[4]   DESIGN OF SEQUENCE-SPECIFIC DNA-BINDING MOLECULES [J].
DERVAN, PB .
SCIENCE, 1986, 232 (4749) :464-471
[5]   THE RNA-FOLDING PROBLEM [J].
DRAPER, DE .
ACCOUNTS OF CHEMICAL RESEARCH, 1992, 25 (04) :201-207
[6]   SEQUENCE-SPECIFIC CLEAVAGE OF SINGLE-STRANDED-DNA - OLIGODEOXYNUCLEOTIDE-EDTA.FE(II) [J].
DREYER, GB ;
DERVAN, PB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (04) :968-972
[7]   3'-TERMINAL LABELING OF RNA WITH T4 RNA LIGASE [J].
ENGLAND, TE ;
UHLENBECK, OC .
NATURE, 1978, 275 (5680) :560-561
[8]  
Gait M. J, 1984, OLIGONUCLEOTIDE SYNT
[9]   STRUCTURE OF AN UNMODIFIED TRANSFER-RNA MOLECULE [J].
HALL, KB ;
SAMPSON, JR ;
UHLENBECK, OC ;
REDFIELD, AG .
BIOCHEMISTRY, 1989, 28 (14) :5794-5801
[10]   SEQUENCE-SPECIFIC RECOGNITION OF DOUBLE HELICAL RNA AND RNA.DNA BY TRIPLE-HELIX FORMATION [J].
HAN, HY ;
DERVAN, PB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (09) :3806-3810
1234