MUTAGENICITY OF BLUE RAYON EXTRACTS OF HUMAN BILE IN THE AMES TEST

被引：8

作者：

MANO, H

YAMAMOTO, M

ARAYA, JC

KATO, K

TSUTSUI, M

OHTA, T

YOSHIDA, K

KINEBUCHI, H

HAYATSU, H

机构：

[1] UNIV LA FRONTERA, DEPT PATHOL, TEMUCO, CHILE

[2] NIIGATA HOSP, CTR PREFECTURAL CANC, NIIGATA, JAPAN

[3] NIIGATA COLL PHARM, DEPT BIOL, NIIGATA 95021, JAPAN

[4] NIPPON DENT UNIV, SCH DENT NIIGATA, DEPT SURG, NIIGATA, JAPAN

[5] KOCHI MED SCH, DEPT ENVIRONM & OCCUPAT HLTH, NANKOKU, KOCHI 78151, JAPAN

[6] OKAYAMA UNIV, FAC PHARMACEUT SCI, OKAYAMA 700, JAPAN

来源：

MUTATION RESEARCH | 1993年 / 290卷 / 02期

关键词：

HUMAN BILE; BLUE RAYON; BILIARY TRACT CANCER; AMES SALMONELLA MICROSOME ASSAY;

D O I：

10.1016/0027-5107(93)90171-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mutagenicity of human bile was examined in the Ames Salmonella/microsome assay. Bile samples were obtained from the gallbladders resected from patients with cholelithiasis, choledocholithiasis, gallbladder cancer, extrahepatic bile duct cancer and other diseases. For extraction of mutagenic components, the bile samples were treated with blue rayon and the adsorbed materials were assayed with Salmonella typhimurium TA98 in the presence of S9 mix. Twenty-four bile samples were tested and positive mutagenic activity was found in 14 samples. A 200-mul bile equivalent material gave 6.3 times as many revertant colonies as the solvent control. With several samples that had undergone two cycles of blue rayon extraction, clear dose-response relationships in mutagenicity were demonstrated.

引用

页码：303 / 309

页数：7