CONCENTRATIONS OF FUMONISIN-B1 IN FEEDS ASSOCIATED WITH ANIMAL HEALTH-PROBLEMS

Ninety-eight samples of feeds associated with 44 cases of equine leukoencephalomalacia (ELEM) and 83 samples of feed associated with 42 cases of a porcine pulmonary edema syndrome (PPE) were analyzed for fumonisin B1, (FB1). For comparison purposes, 51 feed samples not associated with PPE or ELEM were also analyzed. Feed associated with ELEM contained FB1 ranging from less than 1-mu-g/g to 126-mu-g/g with 75% of the cases having at least 1 sample above 10-mu-g/g. Feeds associated with PPE ranged from less than 1-mu-g/g to 330-mu-g/g with 71% of the cases having at least 1 sample greater than 10-mu-g/g. Quantitation was by high performance liquid chromatography (HPLC)/fluorescence using the fluorescamine derivative with confirmation by thin layer chromatography (TLC) and/or gas chromatography/ mass spectroscopy (GC/MS).