EXCHANGEABLE IMMOBILIZED ENZYME REACTOR FOR ENZYME-INHIBITION TESTS IN FLOW-INJECTION ANALYSIS USING A MAGNETIC DEVICE - DETERMINATION OF PESTICIDES IN DRINKING-WATER

被引：83
作者：
KINDERVATER, R
KUNNECKE, W
SCHMID, RD
机构：
[1] GBF - Gesellschaft für Biotechnologische Forschung, Department of Enzyme Technology, D-3300 Braunschweig
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 234卷 / 01期
关键词：
D O I：
10.1016/S0003-2670(00)83545-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A flow-injection system for rapid automated enzyme inhibition testing is described. Exchange of inactivated enzyme immobilized on magnetic particles was performed with magnetic devices which could be electrically switched off to release all bound material. The flow resistance of the reactor was excellent. Inhibition of immobilized acetylcholinesterase was used to determine pesticides in drinking water. Concentrations of 0.5 μg l-1 of the pesticides carbofuran and malaoxon were detected. A complete cycle of analysis, including calibration, took 20 min. © 1990.
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页码：113 / 117
页数：5
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