MOLECULAR-CLONING OF THE COMPLEMENTARY-DNA FOR THE MOUSE PYRUVATE-KINASE M-2 GENE WHOSE EXPRESSION IS DEPENDENT UPON CELL-DIFFERENTIATION

被引：12

作者：

IZUMI, S ^{[1
]}

MANABE, A ^{[1
]}

TOMOYASU, A ^{[1
]}

KIHARANEGISHI, F ^{[1
]}

ARIGA, H ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1267卷 / 2-3期

关键词：

CDNA SEQUENCE; P19; CELL; DIFFERENTIATION; PYRUVATE KINASE;

D O I：

10.1016/0167-4889(95)00071-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding M2-type pyruvate kinase from mouse was cloned and its nucleotide sequence was determined. The cDNA encoded a protein containing 531 amino acids, the nucleotide and amino acid sequences of which were 93.2% and 98.1%, respectively, homologous to those of rat M2 pyruvate kinase. Expression of the pyruvate kinase in mouse embryonal carcinoma P19 cells altered according to differentiation stages; high at undifferentiated and low at differentiated stages.

引用

页码：135 / 138

页数：4