CLONING AND TRANSIENT EXPRESSION OF GENES ENCODING THE HUMAN ALPHA-4 AND BETA-2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR) SUBUNITS

被引：31

作者：

MONTEGGIA, LM

GOPALAKRISHNAN, M

TOUMA, E

IDLER, KB

NASH, N

ARNERIC, SP

SULLIVAN, JP

GIORDANO, T

机构：

[1] ABBOTT LABS,ABBOTT PK,IL 60064

[2] SCIOS NOVA INC,BALTIMORE,MD 21224

来源：

GENE | 1995年 / 155卷 / 02期

关键词：

ION CHANNEL; POLYMERASE CHAIN REACTION; CDNA; SEQUENCE HOMOLOGY; CYTISINE;

D O I：

10.1016/0378-1119(94)00914-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb alpha 4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively, The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain, The 1721-nucleotide beta 2 sequence was identical to the human beta 2 sequence previously reported. Transfection of the alpha 4 and beta 2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [H-3] cytisine, a characteristic of the alpha 4 beta 2 subtype produced in vivo.

引用

页码：189 / 193

页数：5