CLONING AND TRANSIENT EXPRESSION OF GENES ENCODING THE HUMAN ALPHA-4 AND BETA-2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR) SUBUNITS

被引:31
|
作者
MONTEGGIA, LM
GOPALAKRISHNAN, M
TOUMA, E
IDLER, KB
NASH, N
ARNERIC, SP
SULLIVAN, JP
GIORDANO, T
机构
[1] ABBOTT LABS,ABBOTT PK,IL 60064
[2] SCIOS NOVA INC,BALTIMORE,MD 21224
关键词
ION CHANNEL; POLYMERASE CHAIN REACTION; CDNA; SEQUENCE HOMOLOGY; CYTISINE;
D O I
10.1016/0378-1119(94)00914-E
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb alpha 4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively, The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain, The 1721-nucleotide beta 2 sequence was identical to the human beta 2 sequence previously reported. Transfection of the alpha 4 and beta 2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [H-3] cytisine, a characteristic of the alpha 4 beta 2 subtype produced in vivo.
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页码:189 / 193
页数:5
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