CLONING AND TRANSIENT EXPRESSION OF GENES ENCODING THE HUMAN ALPHA-4 AND BETA-2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR) SUBUNITS

被引：31
|
作者：
MONTEGGIA, LM
GOPALAKRISHNAN, M
TOUMA, E
IDLER, KB
NASH, N
ARNERIC, SP
SULLIVAN, JP
GIORDANO, T
机构：
[1] ABBOTT LABS,ABBOTT PK,IL 60064
[2] SCIOS NOVA INC,BALTIMORE,MD 21224
来源：
GENE | 1995年 / 155卷 / 02期
关键词：
ION CHANNEL; POLYMERASE CHAIN REACTION; CDNA; SEQUENCE HOMOLOGY; CYTISINE;
D O I：
10.1016/0378-1119(94)00914-E
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Partial cDNA clones generated by RT-PCR were used as probes to clone the cDNAs encoding the human alpha 4 and beta 2 neuronal nicotinic acetylcholine receptor (nAChR) subunits. The 2.1-kb alpha 4 cDNA shows 84 and 76% identity to the rat and chicken cDNA sequences, respectively, The deduced amino-acid sequence shares 89 and 84% similarity, respectively, with the corresponding rat and chicken proteins, with most of the divergence occurring in the cytoplasmic domain, The 1721-nucleotide beta 2 sequence was identical to the human beta 2 sequence previously reported. Transfection of the alpha 4 and beta 2 clones into HEK293 cells resulted in the formation of binding sites that display high affinity towards [H-3] cytisine, a characteristic of the alpha 4 beta 2 subtype produced in vivo.
引用
收藏
页码：189 / 193
页数：5
相关论文
共 50 条
  • [21] CHROMOSOMAL LOCALIZATION AND PHYSICAL LINKAGE OF THE GENES ENCODING THE HUMAN ALPHA-3, ALPHA-5, AND BETA-4 NEURONAL NICOTINIC RECEPTOR SUBUNITS
    RAIMONDI, E
    RUBBOLI, F
    MORALLI, D
    CHINI, B
    FORNASARI, D
    TARRONI, P
    DECARLI, L
    CLEMENTI, F
    GENOMICS, 1992, 12 (04) : 849 - 850
  • [22] Modulation of recombinant, α2*, α3*or α4*-nicotinic acetylcholine receptor (nAChR) function by nAChR β3 subunits
    Dash, Bhagirathi
    Bhakta, Minoti
    Chang, Yongchang
    Lukas, Ronald J.
    JOURNAL OF NEUROCHEMISTRY, 2012, 121 (03) : 349 - 361
  • [23] Cloning and Spatiotemporal Expression of Zebrafish Neuronal Nicotinic Acetylcholine Receptor Alpha 6 and Alpha 4 Subunit RNAs
    Ackerman, Kristin M.
    Nakkula, Robin
    Zirger, Jeffrey M.
    Beattie, Christine E.
    Boyd, R. Thomas
    DEVELOPMENTAL DYNAMICS, 2009, 238 (04) : 980 - 992
  • [24] Expression of mRNA for neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes
    Kawashima, K
    Sato, KZ
    Fujii, T
    Watanabe, Y
    Yamada, S
    Ando, T
    Fujimoto, K
    JOURNAL OF NEUROCHEMISTRY, 1999, 73 : S142 - S142
  • [25] Alpha4 and Beta2 Subunits of Neuronal Nicotinic Acetylcholine Receptor Genes Are Not Associated with Methamphetamine-Use Disorder in the Japanese Population
    Kishi, Taro
    Ikeda, Masashi
    Kitajima, Tsuyoshi
    Yamanouchi, Yoshio
    Kinoshita, Yoko
    Kawashima, Kunihiro
    Inada, Toshiya
    Harano, Mutsuo
    Komiyama, Tokutaro
    Hori, Toru
    Yamada, Mitsuhiko
    Iyo, Masaomi
    Sora, Ichiro
    Sekine, Yoshimoto
    Ozaki, Norio
    Ujike, Hiroshi
    Iwata, Nakao
    DRUG ADDICTION: RESEARCH FRONTIERS AND TREATMENT ADVANCES, 2008, 1139 : 70 - 82
  • [26] EXPRESSION OF NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR GENES IN THE DEVELOPING CHICK VISUAL-SYSTEM
    MATTER, JM
    MATTERSADZINSKI, L
    BALLIVET, M
    EMBO JOURNAL, 1990, 9 (04): : 1021 - 1026
  • [27] PATCH CLAMP AND PCR ON A SINGLE-RAT RETINAL GANGLION-CELL - NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR) SUBUNITS
    SUCHER, NJ
    DEITCHER, DL
    CEPKO, CL
    LIPTON, SA
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1991, 32 (04) : 992 - 992
  • [28] GENE-EXPRESSION OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR ALPHA-4 SUBUNIT IN THE FRONTAL-CORTEX IN PARKINSONS-DISEASE PATIENTS
    SCHRODER, H
    DEVOS, RAI
    JANSEN, ENH
    BIRTSCH, C
    WEVERS, A
    LOBRON, C
    NOWACKI, S
    SCHRODER, R
    MAELICKE, A
    NEUROSCIENCE LETTERS, 1995, 187 (03) : 173 - 176
  • [29] cSNPs in the genes for the neuronal nicotinic acetylcholine receptor subunits CHRNB2 and CHRNA4.
    Ton, CC
    Kolodziejczak, K
    Zlatanova, D
    Bertucci, CF
    AMERICAN JOURNAL OF HUMAN GENETICS, 2000, 67 (04) : 233 - 233
  • [30] STABLE EXPRESSION AND PHARMACOLOGICAL PROPERTIES OF THE HUMAN ALPHA(7) NICOTINIC ACETYLCHOLINE-RECEPTOR
    GOPALAKRISHNAN, M
    BUISSON, B
    TOUMA, E
    GIORDANO, T
    CAMPBELL, JE
    HU, IC
    DONNELYROBERTS, D
    ARNERIC, SP
    BERTRAND, D
    SULLIVAN, JP
    EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1995, 290 (03): : 237 - 246
12345