ANTIGEN RETRIEVAL FOR BCL-2 PROTEIN IN FORMALIN-FIXED, PARAFFIN-EMBEDDED SECTIONS

被引：25
作者：
UMEMURA, S [1 ]
KAWAI, K [1 ]
OSAMURA, RY [1 ]
TSUTSUMI, Y [1 ]
机构：
[1] TOKAI UNIV HOSP, DIV DIAGNOST PATHOL, ISEHARA, KANAGAWA, JAPAN
来源：
PATHOLOGY INTERNATIONAL | 1995年 / 45卷 / 02期
关键词：
ANTIGEN RETRIEVAL; AUTOCLAVING; BCL-2; PROTEIN; FORMALIN-FIXED PARAFFIN-EMBEDDED SECTIONS; IMMUNOHISTOCHEMISTRY;
D O I：
10.1111/j.1440-1827.1995.tb03429.x
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A commercial monoclonal antibody to bcl-2 protein (bcl-2, 124) is useful in immunohistochemical detection of this 25 kDa oncogene product in frozen sections. In order to overcome the difficulty in identifying bcl-2 protein in formalin-fixed, paraffin-embedded sections, its antigenicity was retrieved by a variety of pretreatments. The heating pretreatment by hydrated autoclaving was the best method for retrieving the antigenicity, and deionized water was the most adequate solution for soaking sections. This antigen retrieval sequence was helpful to examine the localization of bcl-2 protein in archival sections of normal lymphoid and nonlymphoid tissues and follicular lymphomas.
引用
收藏
页码：103 / 107
页数：5
相关论文
共 50 条
[41]   'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues [J].
Fowler, Carol B. ;
Cunningham, Robert E. ;
O'Leary, Timothy J. ;
Mason, Jeffrey T. .
LABORATORY INVESTIGATION, 2007, 87 (08) :836-846
[42]   BCL-2 GENE REARRANGEMENT ANALYSIS OF JAPANESE FOLLICULAR LYMPHOMAS BY POLYMERASE CHAIN-REACTION IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE SPECIMENS [J].
MITANI, S ;
AOKI, N ;
MIZUTANI, S ;
FUJIWARA, M ;
KITAGAWA, T ;
UEHARA, T ;
MORI, S .
JAPANESE JOURNAL OF CANCER RESEARCH, 1993, 84 (01) :37-41
[43]   Immunohistochemical detection of Francisella tularensis in formalin-fixed paraffin-embedded tissue [J].
Guarner, J ;
Greer, PW ;
Bartlett, J ;
Chu, MC ;
Shieh, WJ ;
Zaki, SR .
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 1999, 7 (02) :122-126
[44]   Factors affecting immunoreactivity in long-term storage of formalin-fixed paraffin-embedded tissue sections [J].
Grillo, Federica ;
Pigozzi, Simona ;
Ceriolo, Paola ;
Calamaro, Paola ;
Fiocca, Roberto ;
Mastracci, Luca .
HISTOCHEMISTRY AND CELL BIOLOGY, 2015, 144 (01) :93-99
[45]   Factors affecting immunoreactivity in long-term storage of formalin-fixed paraffin-embedded tissue sections [J].
Federica Grillo ;
Simona Pigozzi ;
Paola Ceriolo ;
Paola Calamaro ;
Roberto Fiocca ;
Luca Mastracci .
Histochemistry and Cell Biology, 2015, 144 :93-99
[46]   Loss of Human Epidermal Receptor 2 Expression in Formalin-Fixed Paraffin-Embedded Breast Cancer Samples and the Rescuing Effect of Enhanced Antigen Retrieval and Signal Amplification [J].
Ma, Xiuli ;
Zhou, Lixin ;
Wu, Qi ;
Jia, Ling ;
Diao, Xinting ;
Kang, Qiang ;
Huang, Xiaozheng ;
Liu, Yiqiang ;
Hu, Taobo ;
Long, Mengping .
LIFE-BASEL, 2024, 14 (01)
[47]   Multiple Protein Analysis of Formalin-fixed and Paraffin-embedded Tissue Samples with Reverse phase Protein Arrays [J].
Assadi, Maziar ;
Lamerz, Jens ;
Jarutat, Tiantom ;
Farfsing, Alexandra ;
Paul, Hubert ;
Gierke, Berthold ;
Breitinger, Ewa ;
Templin, Markus F. ;
Essioux, Laurent ;
Arbogast, Susanne ;
Venturi, Miro ;
Pawlak, Michael ;
Langen, Hanno ;
Schindler, Thomas .
MOLECULAR & CELLULAR PROTEOMICS, 2013, 12 (09) :2615-2622
[48]   Protocol for PTEN Expression by Immunohistochemistry in Formalin-fixed Paraffin-embedded Human Breast Carcinoma [J].
Sakr, Rita A. ;
Barbashina, Violetta ;
Morrogh, Mary ;
Chandarlapaty, Sarat ;
Andrade, Victor P. ;
Arroyo, Crispinita D. ;
Olvera, Narciso ;
King, Tari A. .
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2010, 18 (04) :371-374
[49]   Retinoblastoma susceptibility gene product retrieval in formalin-fixed, paraffin-embedded tissue: A heating method for enhancing immunohistochemical staining [J].
Tsuji, Y ;
Kusuzaki, K ;
Hirasawa, Y ;
Serra, M ;
Baldini, N ;
Campanacci, M .
ACTA HISTOCHEMICA ET CYTOCHEMICA, 1998, 31 (03) :193-196
[50]   Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry [J].
Casadonte, Rita ;
Caprioli, Richard M. .
NATURE PROTOCOLS, 2011, 6 (11) :1695-1709
12345