PREPARATION OF MONOCLONAL-ANTIBODIES FOR IMMUNOBLOTTING HUMAN GROWTH-HORMONE RECEPTOR AND GROWTH HORMONE-BINDING PROTEIN

被引：0
作者：
SAITO, Y
YAMAZAKI, T
SUZUKI, K
IKEBUCHI, H
ASAKURA, A
OTA, Y
SAWADA, J
TERAO, T
机构：
[1] NATL INST HLTH SCI, DIV BIOCHEM & IMMUNOCHEM, 1-18-1 KAMIYOGA, SETAGAYA KU, TOKYO 158, JAPAN
[2] PROT ENGN RES INST, SUITA, OSAKA 565, JAPAN
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1994年 / 17卷 / 07期
关键词：
MONOCLONAL ANTIBODY; IMMUNOBLOTTING; GROWTH HORMONE RECEPTOR; GROWTH HORMONE-BINDING PROTEIN;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal antibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive against hGHRs from human IM-9 cells. The lower limit of detection for immunoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibody also reacted with deglycosylated hGHRs from tunicamycin-treated IM-9 cells and with the growth hormone-binding protein (GH-BP) in human plasma.
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页码：983 / 986
页数：4
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